What are possible interferences in the MLF assay?

Very high alcohol levels may change the response of the system somewhat. The boiling step generally reduces levels far enough to limit this problem. For very high alcohol levels, you can dilute the wine in distilled water to bring the concentration below 10% ABV.
High free SO2 levels (>30ppm) may impact the Biopressure agent. Again, boiling will help this, but if needed you can dilute the wine sample as above.

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