Temperature can have a significant effect on the assay. The recommended temperature is standard room temperature, or about 70 °F (21°C). Temperatures within 3 degrees °F (or 1.5 °C) of this value should be fine. At lower temperatures, the rate of the Biopressure reaction slows down, and the pressure change also is lower, just like car tires lose pressure in cold temperatures. Therefore the assay is less sensitive at lower temperatures.
At higher temperatures, the opposite effect occurs: the reaction will go faster and generate higher values. In principle this is not bad per se – the assay becomes slightly more sensitive with the higher pressures generated. There is nothing wrong with using a higher temperature up to about 95°F (35°C), with two cautions: 1. the higher pressure resulting from higher temperatures may throw off the instrument’s 50 nA set point so it now will signal at a level below the 0.1 g/L level – therefore you will have to pay attention to concentration calculations; 2. most users’ environments aren’t set up to control higher temperatures that well, so increased variability may result. A warm water bath with controlled temperature can be used.
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